|
KMID : 0903620040450020060
|
|
Journal of the Korean Society for Horticultural Science
2004 Volume.45 No. 2 p.60 ~ p.65
|
|
|
Induction and Characterization of Tetraploid Watermelon
|
|
Jaskani, Muhammad J.
Kwon, Sung Whan/Koh, Gab Cheon/Huh, Yun Chan/Ko, Bok Rai
|
|
|
Abstract
|
|
|
|
Diploid watermelon lines were treated with colchicine to develop tetraploid seed parents. Seedlings of ten diploid lines were raised and infected twice with colchicine (0.2, 0.4, and 0.6%) for 3 consecutive days at emergence of true leaves. Watermelon lines responded differently for colchicine concentrations but showed maximum mortality at 0.6% colchicine. The treated seedlings were evaluated on the basis of chloroplast count, DNA content and mortality at 0.6% colchicine. The chloroplast counts in each side of guard cells ranged 5-7 and 10-12 in diploids and tetraploids, respectively. Several plants characterized as tetraploids on the basis on chloroplast count did not prove their tetraploidy on flow cytometric test. The efficacy of treatments indicated that 0.2% colchicine produced more polyploids. However, the recovery of solid tetraploids varied 3.3 to 5.5% in different lines. Tetraploid plants yielded higher leaf area and male flower size than diploids. Pollen morphology showed 3 and 4 colpi in diploids and tetraploids, respectively.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|